Jan 21, 2020 it is also critical to evaluate the role of mammalian glycosylation in altering cellular immunity. Indeed, vaccination of c57bl6 mice with plasmid dna encoding ag85a and ag85b can stimulate strong humoral and cellmediated immune responses and confer significant protection against aerosol or intravenous challenge with live mtb h37rv. Here we have analysed the vaccine potential of a combination of both antigens using plasmid vectors expressing either a fusion protein of both. A novel dna vaccine expressing the ag85aha2 fusion. Figure 1 therapeutic effect of dna vaccines given during chemotherapy on reactivation in a lowdose aerosol infection model.
Construction of a dna vaccine based on the mycobacterium tuberculosis ag85ampt64 fusion gene and evaluation of its immunogenicity. Pdf a novel dna vaccine expressing the ag85aha2 fusion. Tuberculosis dna vaccine encoding ag85a is immunogenic and. Protective effect of dna vaccine during chemotherapy on. Dna vaccines have potential advantages over conventional vaccines, including the ability to induce a. In this study, we developed a dna vaccine encoding the ag85aha2 fusion protein with the aim of inducing protection against both iav and s. Jul 11, 2017 antibody production igg and siga anti ag85a was also significantly increased in bronchoalveolar lavage, as well as in the serum of mice. In this study, mycobacterium tuberculosis mtb rv2190c dna vaccine was prepared and its immunogenicity and therapeutic effects were evaluated. It is also critical to evaluate the role of mammalian glycosylation in altering cellular immunity. Construction of a dna vaccine based on the mycobacterium tuberculosis ag85a mpt64 fusion gene and evaluation of its immunogenicity. In addition, ag85a dna vaccine prevented the reactivation.
The aim of the present work was to investigate the influence of formulating a dna vaccine encoding ag85a with tmc nanoparticles, on the immune responses generated in mice. Longcome biological pharmacy, anhui, china or saline cytokine production in vitro. The aim of the present work was to investigate the in. The immune response of hybrid striped bass morone saxatilis x morone chrysops following oral delivery of a dna vaccine containing mycobacterium marinum ag85a plasmid in either. Protection against mycobacterium tuberculosis challenge in mice by dna vaccine ag85a.
A novel dna vaccine vector encoding the mycobacterium tuberculosis secreted antigen ag85a fused with the influenza a virus iav ha2 protein epitopes, pegfpag85asha2 pag85asha2, was designed to provide protection against influenza. A novel tuberculosis dna vaccine in an hiv1 p24 protein. Chapter 3 immunogenic and protective effects of a dna. Secondary pneumonia due to staphylococcus aureus s. Immunogenicity and protective efficacy of tuberculosis dna. When the dna vaccine is administered to a patient, the machinery in their cells makes a viral or bacterial protein which their immune system. Immunizing the host with a piece of viral dna rather than an antigenic protein fragment of the virus, helps to stimulate the generation of cellmediated immunity fig. Vaccines are considered to be the most costeffective strategy to.
Because preparation of mixed dna vaccines is troublesome, not. Tuberculosis dna vaccine encoding ag85a is immunogenic. Immunizing the host with a piece of viral dna rather than an antigenic protein fragment of the virus, helps to stimulate. Immunogenicity of a dna vaccine encoding ag85atb10. Dna vaccine expression of il2 and the hsp65 fusion gene was studied. Dna vaccination is the method of choice for the induction of protective th1 type immune responses with the ag85a tuberculosis dna vaccine. The length of gene sequence encoding for strong immunogen ag85a was 888 bp, and the ag85b gene sequence was 474 bp. May 26, 2006 indeed, vaccination of c57bl6 mice with plasmid dna encoding ag85a and ag85b can stimulate strong humoral and cell. When the dna vaccine is administered to a patient, the machinery in their cells makes a viral or bacterial protein which their immune system recognises as being foreign to the body.
In order to explore whether ag85a dna vaccine could mount more efficiently protective immune response through new rna sensor and its signal transduction pathway of antigen presentation we designed and synthesized ag85a gene fragment containing multiple points mutations and transfected the gene fragment into the dendritic cell line dc2. A novel dna vaccine vector encoding the mycobacterium tuberculosis secreted antigen ag85a fused with the influenza a virus iav ha2 protein epitopes, pegfp ag85a sha2 pag85asha2, was designed to provide protection against influenza. Tuberculosis tb is a major global public health problem. Mycobacterium tuberculosis ag85ab chimeric dna vaccine was thus. To design a novel multivalent dna vaccine against m.
This study demonstrated that a dna vaccine encoding the m. Mar 01, 20 use of therapeutic dna vaccines is a promising strategy against tb. Molecular adjuvant ag85a enhances protection against. Previous studies have demonstrated that dna vaccines exert profound therapeutic effects in mice with tb 5,6. Mycobacterium tuberculosis ag85ab chimeric dna vaccine was thus constructed by inserting the sequence encoding amino acids 125282 of ag85b protein into nucleotide 430435 endonuclease enzyme acc i site of ag85a dna vaccine. Noninfected mice immunized with rv2190c dna or ag85a dna showed higher levels of interferongamma ifn. Jan 31, 20 secondary pneumonia due to staphylococcus aureus s. Staphylococcus aureus, influenza a viruse, mycobacterium tuberculosis secreted antigen ag85a, dna vaccine introduction secondary pneumonia due to staphylococcus aureus s. Immunogenicity and therapeutic effects of ag85ab chimeric dna. Designing and construction a dna vaccine encoding the. Chapter 3 immunogenic and protective effects of a dna vaccine.
Dna vaccines can stimulate both humoral and cellmediated immunity in different animal models of tb and is thought to be a promising strategy in the development of new vaccines against tb. The aim of the research was designed a novel dna vaccine encoding the mycobacterium tuberculosis secreted antigen ag85a fused with the influenza a virus iav ha2 protein to provide protection against both influenza and secondary infection with s. It elevated the immunogenicity and protective as well as therapeutic effects of the hsp65 dna vaccine against tb in mice. Ag85aesat6 chimeric dna vaccine induces an adverse. Dou department of pathogenic biology and immunology, medical school, southeast university, nanjing, 29 china. Dna vaccines expressing the individual antigens were either beneficial or at least. Fingerling hybridstriped bass were obtained from a commercial supplier and housed at the aquatic medicine laboratory of the vmrcvm in. A novel dna vaccine expressing the ag85aha2 fusion protein provides protection against influenza a virus and staphylococcus aureus. Mechanisms and aspects of relevance for biosafety biosafety first 2007 traavik, t.
Therapeutic effect of dna vaccines combined with chemotherapy in. Use of therapeutic dna vaccines is a promising strategy against tb. In the study, we developed the dna vaccine encoding fusion protein of antigen 85 a and 6 kda early secretory antigen target of m. Another method to improve dna vaccine immunogenicity is the inclusion of additional plasmids, or additional inserts in the same plasmid, encoding molecular adjuvants. Influence of the formulation characteristics on immune responses by johanna poecheim 1, christophe barnierquer 2, nicolas collin 2 and gerrit borchard 1. The mechanisms of ag85a dna vaccine activates rna sensors. In light of these findings, it is imperative to investigate the potential impact of mammalian glycosylation on cellmediated immune responses. Liu, in encyclopedia of immunology second edition, 1998. Improved protective efficacy of a speciesspecific dna vaccine. Combination chemotherapy is very effective in curing this disease but, unfortunately, the. In addition, these antigens represent vaccine components that were tested in early human clinical trials. Dna vaccination is a technique for protecting against disease by injection with genetically engineered plasmid containing the dna sequence encoding the antigens against which an immune response is sought so cells directly produce an antibody, producing a protective immunological response. Methods and protocols, third edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of dna vaccines. Immunogenicity and therapeutic effects of a mycobacterium tuberculosis rv2190c dna vaccine in mice yan liang1, xiaoyan zhang1,2, xuejuan bai1, li xiao1, xiaomei wang1, junxian zhang1, yourong yang1, jinying song1, lan wang1 and xueqiong wu1 abstract background.
Isolation, cloning and molecular analysis of ag85a and. A dna vaccine uses a gene from a virus or bacteria to stimulate the immune system. Briefly, peinp coated dna vaccine piresil21 ag85a esat6 or pires mock plasmid was prepared by shaking the mixtures of 5 ml of the nanofe 3 o 4glu to 1 ml pei 5. It elevated the immunogenicity and protective as well as therapeutic effects of the hsp65dna vaccine against tb in mice. The research presented here successfully created an ag85a dna vaccine that stimulated protective immunity in hybridstriped bass against m. Briefly, peinp coated dna vaccine piresil21ag85aesat6 or pires mock plasmid was prepared by shaking the mixtures of 5 ml of the nanofe 3 o 4glu to 1 ml pei 5. The aim of this study was to design and construct a dna vaccine encoding ag85a and tb10. Research open access a novel dna vaccine expressing the. In the present study, a chimeric dna vaccine was constructed that.
Materials and methods plasmid construction and dna vaccine preparation. Vaccination with plasmid dna encoding ag85a from m. Aug 01, 2011 an influenza dna vaccine formulated in the lipid compound vaxfectin vical induced protective antibody titers and tcell responses in many subjects. In summary, these findings open new perspectives in the area of mucosal dna vaccine, against specific pathogens using a. A novel dna vaccine expressing the ag85aha2 fusion protein. Based on this hypothesis, the mice in this study were vaccinated with a dna vaccine encoding ag85a and tb10. Immunogenicity and protective efficacy of a dna vaccine encoding ag85a from mycobacterium tuberculosis were compared in balbc and c57bl b6 and b10. Tuberculosis remains a major health problem affecting millions of people worldwide 5. Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on dna vaccine design and enhancement, delivery systems, production, purification, and quality. After confirming that they could express successfully in mammalian cells, the novel vaccine and respective controls were used to immunize mice by the intramuscular route before challenge with iav and s. Antibody production igg and siga antiag85a was also significantly increased in bronchoalveolar lavage, as well as in the serum of mice. Tuberculosis tb is one of most important chronic infectious diseases caused by mycobacterium tuberculosis and remains a major global health problem. Evaluation of immune responses to a dna vaccine encoding.
Immunogenicity and therapeutic effects of ag85ab chimeric. Immunogenic and protective effects of a dna vaccine for. May 05, 2020 human trials testing dna vaccines against influenza virus, malaria, hepatitis b virus, hiv, herpes simplex virus, colon cancer, and cutaneous tcell lymphoma are underway and have only just begun. Xueqiong wu, yan liang, junxian zhang, li xiao, yourong yang, xuejuan bai, qi yu, zhongming li and ning li. Dna vaccine prepared using recombinant flagellin protein derived from salmonella as mucosal adjuvant for anticaries dna vaccine. Immunogenicity and therapeutic effects of a mycobacterium. In summary, these findings open new perspectives in the area of mucosal dna vaccine, against specific pathogens using a lactic acid bacteria such as l. Vaccines free fulltext ag85a dna vaccine delivery by. These results demonstrated that ag85a could strengthen the immune response to iav and s. This was achieved by improving the th1type response34. Comparison of bcg primedna booster and rbcg regimens.
Isolation, cloning and molecular analysis of ag85a and tb10. Research open access a novel dna vaccine expressing the ag85a. In balbc mice, gg immunization could induce elevated antibody and cytotoxic t lymphocyte responses with plasmid. A multicistronic dna vaccine induces significant protection. Influence of the formulation characteristics on immune responses. Construction and immunogenicity of a39 dna vaccine against. The influence of dna vaccine formulations on immune responses in combination with adjuvants was investigated with the aim to increase cellmediated immunity against plasmid dna pdna encoding mycobacterium tuberculosis antigen 85a. An influenza dna vaccine formulated in the lipid compound vaxfectin vical induced protective antibody titers and tcell responses in many subjects. Dna vaccines is a major updated and enhancement of the first edition. In balbc mice, gg immunization could induce elevated antibody and cytotoxic t lymphocyte responses with plasmid doses 50fold lower than those. August 2007,dna vaccination against sclerosis was reported as being effective. Designing and construction a dna vaccine encoding the fusion. August 2007, dna vaccination against sclerosis was reported as being effective. The pei modified dna vaccine was performed as described previously 19, 20.
A dna vaccine was constructed using the mycobacterium marinum ag85a gene, which was isolated and subcloned into a commerciallyavailable eukaryotic expression vector. The study on dna vaccines first began in the 1990s, when the plasmid dna is injected into the skin or muscle was reported to induce antibody responses to antigens. The bcg and the control groups received bcg and pbs vaccines, respectively. Dec 19, 20 horse acquired immunity against west nile viruses. Construction of a dna vaccine based on the mycobacterium. Therapeutic effect of dna vaccines given during chemotherapy on reactivation in a lowdose aerosol infection model. Immunogenicity and protective efficacy of a dna vaccine encoding ag85a from mycobacterium tuberculosis were compared in balbc and c57bl b6 and b10 mice immunized by intramuscular i. Effects of the fusion design and immunization route on the. This model has been instructive, in that in this system the mycobacterial protein is made by the mammalian host cell rather than by the organism. Human trials testing dna vaccines against influenza virus, malaria, hepatitis b virus, hiv, herpes simplex virus, colon cancer, and cutaneous tcell.
Ag85a gene was firstly amplified by polymerase chain reaction pcr and then cloned into pvax1 to construct the recombinant plasmid pvax1ag85a a. Furthermore, the degree of immune responses elicited appeared roughly proportional to the amount of protection conferred. Request pdf immunogenicity and therapeutic effects of ag85ab chimeric dna vaccine in mice infected with mycobacterium tuberculosis the situation of tuberculosis tb is very severe in china. Host protein glycosylation in nucleic acid vaccines as a. Pdf ag85aesat6 chimeric dna vaccine induces an adverse.
Ag85aesat6 chimeric dna vaccine induces an adverse response. Protection against mycobacterium tuberculosis challenge in. Sep 12, 2016 the aim of the present work was to investigate the influence of formulating a dna vaccine encoding ag85a with tmc nanoparticles, on the immune responses generated in mice. Dna vaccines encoding the 32 000 mw mycolyltransferase ag85a and the 40 000 mw phosphatebinding protein psts3 can elicit protective immune responses against experimental infection with mycobacterium tuberculosis in c57bl6 mice. Accordingly, dna encoding ag85a is currently contemplated as a promising dna vaccine candidate against mtb due to its ability to stimulate humoral and cellmediated immune responses in mice, as well as protection in mice against mtb challenge. Pgacoated complex to pubm, a melanoma dna vaccine, noticeably impeded the growth and metastasis of a melanoma cell line, b16f10 cells. Methods and protocols, third edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of dna vaccines featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on dna vaccine design and enhancement, delivery systems, production, purification, and. A dna vaccine encoding ag85acfp10 fusion protein was. Recombinant invasive lactococcus lactis carrying a dna.
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